Objective
BACKGROUND: Proteomics is a high-throughput approach that could provide comprehensive understanding of complex developmental processes. Holm oak (Quercus ilex L.) is a dominant tree in Mediterranean natural communities. Oak seeds are recalcitrant. Seed proteome and germination under stress are studied predominantly in cereals and model plants, but there is very limited information on forest species.
OBJECTIVES: This project aims to study germination and early seedling growth of Holm oak at the proteome level, focusing particularly on how these processes are affected by drought. Emphasis will be placed on phosphorylation and ubiquitination as major posttranslational modifications (PTM). Proteome analysis will be complemented with data at the metabolite and transcript levels and with cytochemical observations.
METHODOLOGY: Holistic and targeted approaches will be combined.
Holistic approach – by using gel and gel-free, including label free quantitation approaches coupled to MS, changes in the protein and PTM profiles associated with the germination and stress responses will be analyzed. PTM will be detected by specific staining or Western blot. Appropriate statistical analysis, algorithms, databases and repositories will be used.
Targeted approach – the oxidative status of the tissues will be monitored and more detailed analysis of some proteins of interest will be performed at the transcript level. Analyses at the metabolite level will include carbohydrates (ethanol extraction, GC-MS), aminoacid derivates (ethanol extraction, HPLC quantification), some secondary metabolites (GC or HPLC). Histochemical staining of carbohydrates, phenolic compounds, and H2O2 will also be performed.
EXPECTED RESULTS: To gain deeper knowledge of the response to drought in a recalcitrant seed- producing tree species at the earliest developmental stages. The relevance of specific PTMs will be evaluated. Proteomic data will be validated by complementary targeted studies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences computer and information sciences databases
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- natural sciences chemical sciences organic chemistry alcohols
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IEF
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
14005 CORDOBA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.