The mechanism of retrograde trafficking by retromer

Projektinformationen

RETROMER

ID Finanzhilfevereinbarung: 277078

Abgeschlossenes Projekt

Startdatum 1 März 2011

Enddatum 28 Februar 2015

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 100 000,00

EU-Beitrag € 100 000,00

Koordiniert durch

INSTITUT CURIE
France

Ziel

Transportation of proteins and lipids between membrane-enclosed organelles by vesicle formation is a fundamental task in eukaryote cells. The least understood protein complex involved in membrane remodelling for vesiculation, but with a wide range of medical implications, is the retromer complex.

The objective of this proposal is:
First, to identify the mechanistic principles of membrane bending and scission by retromer, and second to characterize temporal and morphological aspects of retromer and cargo molecules (Shiga toxin (STx) and Alzheimer’s amyloid-beta-cleaving enzyme (BACE)).

I will exploit two approaches:
First, in vitro reconstitution of the retromer complex using giant unilamellar vesicles to deploy biophysical methods.
Second, I will provide a molecular choreography and anatomy of retrograde transport of STx, BACE and retromer by implementing a new imaging technique, structured-illumination microscopy combined with single particle tracking.

The proposed interdisciplinary research will break new ground for membrane mechanics and trafficking of disease related cargo molecules.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › neurology › dementia › alzheimer
• medical and health sciences › basic medicine › anatomy and morphology
• natural sciences › physical sciences › optics › microscopy
• natural sciences › biological sciences › biochemistry › biomolecules › lipids
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2010-RG
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IRG - International Re-integration Grants (IRG)

Koordinator

INSTITUT CURIE

Kontakt Verwaltung

Corinne Cumin (Ms.)

• EU-Beitrag: € 100 000,00
• Adresse: RUE D ULM 26 ; 75231 Paris ; Frankreich
• Region: Ile-de-France › Ile-de-France › Paris
• Aktivitätstyp: Research Organisations
• Gesamtkosten: Keine Daten