Objective
There are aminoacyl transferases, such as arginyl tRNA protein transferase 1 (Ate1) in Eukaryotes and leucyl/phenylalanyl tRNA protein transferase (L/F transferase) in Bacteria. Both enzymes recognize specific N-terminal amino acids of a target poly-peptide and conjugate an amino acid from an aminoacylated tRNA onto the N-terminus of the targeted poly-peptide.
Ate1 conjugates an arginyl moiety onto the N-terminus of a poly-peptide. Electrospray ionization of peptides critically depends on arginyl moieties to stabilize proton charges to the peptide. Taking both facts together, there is a huge untapped potential for Ate1 in the workflow of mass spectrometry facilities by providing an additional proton charge-stabilizing moiety to a peptide's N-terminus.
With my proposed project I further aim to harvest the ability of L/F transferase to conjugate a single amino acid, including unnatural amino acids, to the N-terminus of specific target poly-peptide and covalently tag the N-terminus of proteins. The proteins of interest are proteolysis fragments, which will be positively enriched by taking advantage of the unnatural amino acid moiety and analyzed using mass spectrometry. The monitoring of proteolysis products has far reaching implications as proteolysis is an important causal or progression factor in various diseases such as neurodegenerative diseases, chronic inflammation, cancer and heart disease.
During my post-doctoral studies at the University of Alberta, Canada, I characterized the kinetic parameters of both L/F transferase and Ate1 extensively. As Marie Curie International Incoming Fellow, I will build on my previous knowledge in a team of biochemists, cell biologists, computational biologists, and mass spectrometrists to establish sound methodologies for clinical and basic researchers alike and will assist in transferring newly developed methodologies in other mass spectrometry facilities in Europe.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences microbiology bacteriology
- medical and health sciences clinical medicine oncology
- natural sciences chemical sciences organic chemistry amines
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IIF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
8092 Zurich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.