Objectif We propose to develop a microfluidic chip-sized assay for low-cost, sensitive, rapid, and multiplexed detection of bacteria in urine. We aim to reduce bacterial diagnostic time from the current standard of 1-3 days to several minutes. The device will have application to both conventional clinical microbiology laboratories and point-of-care settings, and will eliminate dependence on highly trained operators. We propose to use isotachophoresis (ITP) to extract and concentrate the bacterial signature 16S ribosomal RNA directly from urine samples, and detect and quantify its presence using a set of complementary fluorescent probes (molecular beacons) on a low-cost microfluidic chip. Champ scientifique natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesclinical medicineurology Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Appel à propositions FP7-PEOPLE-2011-CIG Voir d’autres projets de cet appel Régime de financement MC-CIG - Support for training and career development of researcher (CIG) Coordinateur TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY Contribution de l’UE € 100 000,00 Adresse SENATE BUILDING TECHNION CITY 32000 Haifa Israël Voir sur la carte Type d’activité Higher or Secondary Education Establishments Contact administratif Mark Davison (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
