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Final Report - BIOSYN-NUDICAUL (Biosynthetic studies of nudicaulins in laser-microdissected petal cell of Papaver nudicaule by the use of NMR spectroscopy and hyphenated technique LC-NMR)

Project ID: 221274
Funded under: FP7-PEOPLE

Abstract

The major objectives of the BIOSYN-NUDICAUL project are summarised to the following:
- elucidation of the nudicaulin biosynthesis;
- distribution of nudicaulins in plant kingdom;
- investigation of their ecological role and physiological function;
- potential pharmacological use

To achieve the objectives of the project the following tasks were performed:
- growing plants under controlled conditions and the study of their development;
- establishment of conditions for in vitro cultures of papaver nudicaule plants;
- development of an analytical protocol for analysis of plant extract and isolation of target compounds;
- incubation of Iceland's poppy plants with supplemental Ultraviolet-B (UV-B) radiation and phytochemical analysis of lower petals;
- metabolic profile of papaver nudicaule plant organs during the development of plant;
- hydrolysis of nudicaulins under basic and acidic conditions for removal of malonyl and glucosidic conjugations, isolation of demalonylated nudicaulins and aglyca;
- bioassay of isolated nudicaulins for pharmacological activities;
- administration of 13C labelled precursors to buds from Iceland's poppy plants;
- pulse / chase labelling experiments with 13CO2 followed by isolation of primary and targeted secondary metabolites;
- retrobiosynthetic analysis of Nuclear magnetic resonance (NMR) data;
- comparative genomic and proteomic analysis.

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Related information

Record Number: 10582 / Last updated on: 2010-12-31
Category: PROJ
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