CHROMOSOME ANALYSIS AND MICRONUCLEUS TEST IN MOUSE BONE MARROW ARE NOT EQUALLY SENSITIVE AS SHOWN BY RESULTS WITH 5 CHEMICAL MUTAGENS
The two bone-marrow tests - chromosome analysis and micronucleus test - are recommended by the EEC and OECD guidelines for mutagenicity testing as alternative procedures for cytogenetic screening in vivo. These recommendations imply that the two tests are equally sensitive. Previous data with mitomycin C, methyl methanesulfonate and procarbazine supported that Further experiments to compare the sensitivity of the two tests were performed with mice using benzo[a]pyrene (B[a]P), ethylmethanesulfonate (EMS), ethylnitrosourea (ENU), atrazine and epichlorhydrin. The interval of maximum response was determined for each test and the lowest effective doses were determined at that particular time with each chemical. Otherwise the tests were performed under identical conditions. The present data indicate that the in vivo chromosome analysis is more sensitive than the micronucleus test. Based on the results with these 5 chemicals it seems questionable that the two tests are true alternatives to screen for possible clastogenic effects of a chemical under test. In view of economical considerations the micronucleus test could be preferred in the initial step of a screening protocol. If the result is negative a test for metaphase analysis in vivo should be recommended in the next phase of testing.
Bibliographic Reference: MUTATION RESEARCH, VOL. 113 (1983), PP. 340-341
Record Number: 1989122014200 / Last updated on: 1987-01-01
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