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Abstract

The main properties and functions of the DNA polymerases alpha, beta and gamma in eukaryotic cells are described. Their activities can be distinguished by menas of specific assays. Taking into account biochemical aspects and the sensitivity or resistance to inhibitors, new definitions are proposed. In order to study more precisely the structure of these three enzymes, new techniques are adopted which allow the direct visualization of the peptides belonging to the DNA polymerases after separation by SDS polyacrylamide gel electrophoresis, starting from the crude extracts of various organisms. Results, related to mammalian cells, insect cells and embryos, bacteria cells, fungi and plant cells extracts, are presented and discussed.

Additional information

Authors: BERTAZZONI U, ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY);JRC ISPRA ESTAB. (ITALY);TORSELLO S, ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY);JRC ISPRA ESTAB. (ITALY);SCOVASSI A I, ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY);JRC ISPRA ESTAB. (ITALY);CAVALLORO R ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY), ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY);JRC ISPRA ESTAB. (ITALY);JRC ISPRA ESTAB. (ITALY), ISTITUTO C.N.R. GENETICA BIOCHIMICA EVOLUZIONISTICA, PAVIA (ITALY);JRC ISPRA ESTAB. (ITALY)
Bibliographic Reference: MEETING ON CANCER RISK AND DNA REPAIR, ROMA (ITALY), FEB. 23, 1982 - WRITE TO CEC LUXEMBOURG, DG XIII/A2, POB 1907 MENTIONING PAPER E 31385 ORA
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