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Abstract

We examined the applicability and reliability of the ELISA method for the detection of group and interspecific determinants in p30 proteins of mammalian C-type retroviruses. The specificity of the observed reaction was further demonstrated by competition ELISA, by pre-absorption of the coating antibody with homologous and heterologous antigen, and by ELISA with F (ab')-2 fragments of the IgG molecules. After exclusion of rheumatoid factor (RF) interference, the immunoaffinity purified human antigen was characterized by SDS polyacrylamide gel electrophoretic analysis, enzymatic digest, and peptide maps. The antigen found in human leukemic sera is a protein distinct from commmon serum proteins.

Additional information

Authors: SCHETTERS H, MEDIZINISCHE POLIKLINIK DER UNIVERSITAET MUENCHEN (GERMANY);GESELLSCHAFT FUER STRAHLEN- UND UMWELTFORSCHUNG MBH MUENCHEN, NEUHERBERG (GERMANY);ERFLE V, MEDIZINISCHE POLIKLINIK DER UNIVERSITAET MUENCHEN (GERMANY);GESELLSCHAFT FUER STRAHLEN- UND UMWELTFORSCHUNG MBH MUENCHEN, NEUHERBERG (GERMANY);HEHLMANN R MEDIZINISCHE POLIKLINIK DER UNIVERSITAET MUENCHEN (GERMANY), MEDIZINISCHE POLIKLINIK DER UNIVERSITAET MUENCHEN (GERMANY);GESELLSCHAFT FUER STRAHLEN- UND UMWELTFORSCHUNG MBH MUENCHEN, NEUHERBERG (GERMANY);GESELLSCHAFT FUER STRAHLEN- UND UMWELTFORSCHUNG MBH MUENCHEN, NEUHERBERG (GERMANY), MEDIZINISCHE POLIKLINIK DER UNIVERSITAET MUENCHEN (GERMANY);GESELLSCHAFT FUER STRAHLEN- UND UMWELTFORSCHUNG MBH MUENCHEN, NEUHERBERG (GERMANY)
Bibliographic Reference: PP. 284-288, PUBLISHED IN HAEMATOLOGY AND BLOOD TRANSFUSION, VOL. 28 (1983), MODERN TRENDS IN HUMAN LEUKEMIA V, EDITED BY SPRINGER-VERLAG (GERMANY)
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