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Abstract

The composition of dog fetal liver with respect to lymphocyte content and immune responsiveness of single cell suspensions in vitro was investigated by histology, immunofluorescence (B cells), lymphocyte transformation (LT), and mixed leukocyte culture (MLC). Lymphocytes were extremely rare in organ sections by day 39 of gestation and became more numerous close to term. In parallel, B cell proportions increased in single cell suspensions from less than 0.1 degrees of non-hepatocytic cells by day 39 to about the 5 degrees one day post partum (day 64). No proliferation occurred upon mitogen stimulation with PHA, Con A, or PWM throughout gestation, MLC responses could be detected, however, by day 34, decreasing to low levels between days 41 and 50, and rising again thereafter. Early responses were brought about by proliferation of hemopoietic progenitor cells and their progeny. No suppression was exerted by 45 day liver cells on adult mononuclear cells in LT or MLC assays. Thus, mechanisms different from active suppression may account for the weak responsiveness of liver cells at this developmental stage. With regard to transplantation purposes, fetal liver grafts up to gestation day 50 might prove capable of reconstituting hemopoiesis in allogeneic recipients without ensuing acute graft-versus-host disease.

Additional information

Authors: PRUEMMER O, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);CALVO W, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);FLIEDNER T M, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);NOTHDURFT W DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY), DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY)
Bibliographic Reference: RECENT ADVANCES IN BONE MARROW TRANSPLANTATION, 1983, PP. 841-848, PUBLISHED BY ALAN R. LISS, INC., 150 FIFTH AVENUE, NEW YORK, NY 10011 (USA)
Record Number: 1989122025600 / Last updated on: 1987-01-01
Category: PUBLICATION
Available languages: en