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  • REPORT OF THE SECOND STAGE IN THE DEVELOPMENT OF A STANDARDIZED LABORATORY METHOD FOR ASSESSING THE TOXICITY OF CHEMICAL SUBSTANCES TO EARTHWORMS

Abstract

The interlaboratory intercalibration ring test assessing the validity and reproducibility of proposed contact filter paper, artificial soil and an "artisol" toxicity test for the earthworm E. foetida< in 1981 - 1982 involved the assessment of the toxicity of pentachlorophenol, carbaryl, trichloroacetic acid (unknown test chemicals) and copper sulphate, by 60 laboratories in 18 countries. The order of toxicity were pentachlorophenol > carbaryl < copper sulphate > trichloroacetic acid. The contact test gave the most reproducible results, was difficult to interpret and the data gave only potential toxicity hazards, the artificial soil test, although slightly less precise and rather more time consuming, gave much more readily interpreted data. The 1982 - 1983 intercalibration test assessed the toxicities of a new reference standard chloracetamide, also pentachlorophenol, potassium bromide and technical chlordane. The order of toxicities was chloracetamide > technical chlordane < pentachlorophenol < potassium bromide. Nearly all results varied by less than an order of magnitude. Insufficient results were received on the "artisol" test. Selection of the most suitable method(s) should now be relatively easy.

Additional information

Authors: EDWARDS C A ROTHAMSTED EXPERIMENTAL STATION, HARPENDEN, HERTS. (UK), ROTHAMSTED EXPERIMENTAL STATION, HARPENDEN, HERTS. (UK)
Bibliographic Reference: EUR 9360 EN (1984) MF, 102 P., BFR 240, BLOW-UP COPY BFR 510, EUROFFICE, LUXEMBOURG, POB 1003
Availability: Can be ordered online
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