DIRECT DETERMINATION OF LEAD IN BLOOD BY LASER EXCITED ATOMIC FLUORESCENCE FLAME SPECTROMETRY
A simple method for the determination of lead in blood by atomic fluorescence is described where a dilution 1 to 10 with Triton-X is carried out on the sample which is then nebulized directly into an argon separated air - acetylene flame burning on a circular, home made, capillary burner. Aqueous standards can be used for calibration purposes. The direct line fluorescence of lead at 405.8 nm is measured after excitation at 283.3 nm provided by a frequency doubled pulsed tunable dye laser pumped by a XeC1 excimer laser, thus avoiding any scattering due to the matrix. The laser peak power was approximately 40 kW at the uv wavelength and the fluorescence was highly saturated even when the beam size was expanded in the flame to 15 mm diameter. By diluting 0.1 ml of blood to 1 ml and using discrete sampling in the flame, the detection limit was found to be 4 ng/ml and can be decreased considerably by taking a larger volume of blood. Several blood samples as well as quality control samples were analyzed and the results found in very close agreement with those provided by the well established Delves cup atomic absorption, graphite furnace atomic absorption, graphite furnace atomic absorption and anodic stripping voltammetry procedures.
Bibliographic Reference: THE ANALYST, VOL. 109 (1984), PP. 1067-1070
Record Number: 1989123000800 / Last updated on: 1987-01-01
Available languages: en