SYNTHESIS OF DNA FRAGMENTS
The chemical synthesis of oligodeoxynucleotides can be achieved in laboratory by three distinct techniques: the phosphate diester, the phosphate triester and the phosphite triester method. The first procedure envisages the chemical combination of two nucleotides in the presence of a condensing agent. The second method differs from the preceding one by the presence of an additional protecting group in the phosphate. The third method relies on the combination of a nucleoside or a nucleotide with a phosphite nucleoside in order to synthesize an internucleotide phosphite ester bridge. This linkage is subsequently oxidized to the phosphate level so as to restore the normal DNA structure. The phosphate triester procedure is faster than the others and it is easily adapted to the stepwise polymerization of deoxynucleotides on a solid support. The solid-phase synthesis facilitates greatly the manipulations needed to isolate the products. Biochemical methods for synthesizing oligodeoxynucleotides with base specific sequence and DNA-like polymer for enzymatic studies will be discussed.
Bibliographic Reference: CONVEGNO-SCUOLA SU BIOPOLIMERI, GARGNANO, BRESCIA (ITALY), JUNE 4-8, 1984 WRITE TO CEC LUXEMBOURG, DG XIII/A2, POB 1907 MENTIONING PAPER E 31678 ORA
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Record Number: 1989123001400 / Last updated on: 1987-01-01
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