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Abstract

The photosynthetic bacterium, Rhodopseudomonas capsulata, exhibits a vigorous photoevolution of H-2 under certain conditions. The use of cultures, or immobilized cells, of this organism in a bioreactor, is therefore envisaged as a means of solar energy conversion. The purpose of the present research is to characterize physiologically, biochemically and genetically the H-2 evolving (nitrogenase) and H-2 uptake (hydrogenase) systems of Rhodopseudomonas capsulata in order to growth and reaction conditions and genetic characteristics, which are optimal for H-2 production. We have studied in detail the effect of light intensity, N source and pH on the production of H-2 by continuous cultures. The role of the uptake hydrogenase in the recycling of H-2 and in the respiratory protection of nitrogenase has also been considered and has been studied as follows: 1- isolation and biochemical characterization of hydrogen uptake deficient (Hup**-) mutants; 2- comparison of the efficiencies of H-2 production by the Hup**- mutants and wild strain B10; 3- analysis of the relationship between hydrogenase activity and nitrogenase activity in continuous culture. Finally, the use of whey as a cheap substrate for H-2 production has been evaluated. Assuming optimal rates of H-2 production, the estimated cost of H-2 produced by this system compares favourably with that of H-2 produced by electrolysis.

Additional information

Authors: VIGNAIS P M, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);WILLISON J C, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);COLBEAU A, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);YOUANNEAU Y, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);MADERN D, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);HALLEBBECK P C, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);THIERRY J P, DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE);RENAUD M DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE), DEPARTEMENT DE RECHERCHE FONDAMENTALE, CENTRE D'ETUDES NUCLEAIRES, GRENOBLE (FRANCE)
Bibliographic Reference: EUR 9440 EN (1984) MF, 27 P., BFR 120, BLOW-UP COPY BFR 150, EUROFFICE, LUXEMBOURG, POB 1003
Availability: Can be ordered online
Record Number: 1989123017200 / Last updated on: 1987-01-01
Category: PUBLICATION
Available languages: en