Community Research and Development Information Service - CORDIS

Abstract

The primary structure of nucleic acids is currently determined by direct methods revealing the specific distribution of the various nucleotide units along the chain-like linear polymer. The base sequence of the homogeneous nucleic acid samples is reconstructed from the analysis of the gel electrophoretic patterns obtained with fragments or fragment equivalents of the polymer that differ in length by a single nucleotide. Nested fragments of RNA are obtained by base specific enzymatic digestion with the aid of selective RNAses. Cleavage of DNA chain at base specific sites is induced by selective chemical modification of either purine or pyrimidine residues, followed by reactions that cause the release of the altered bases and the opening of the sugar phosphate backbone of the polymer. Alternatively, the base sequence of DNA can be deduced by analysis of the products recovered from the complementary enzymatic duplication of a DNA template molecule in the presence of base specific terminators of the polymerization catalysis.

Additional information

Authors: CAMPAGNARI F JRC ISPRA ESTAB. (ITALY), JRC ISPRA ESTAB. (ITALY)
Bibliographic Reference: CONVEGNO-SCUOLA SU BIOPOLIMERI, GARGNANO, BRESCIA (ITALY), JUNE 4-8, 1984 WRITE TO CEC LUXEMBOURG, DG XIII/A2, POB 1907 MENTIONING PAPER E 31677 ORA
Availability: Can be ordered online
Record Number: 1989123026700 / Last updated on: 1987-01-01
Category: PUBLICATION
Available languages: en