IMMOBILIZATION OF HYDROGENASE SYSTEMS FOR THE PHOTOCHEMICAL, PHOTOVOLTAIC AND PHOTOBIOLOGICAL PRODUCTION OF HYDROGEN
New methods were investigated for the production of hydrogen by light. The systems investigated made use of the properties of the enzyme hydrogenase in its reaction with a reduced electron donor (artifical or natural) to produce hydrogen. The hydrogenases were the purified enzymes from Desulfovibrio vulgaris or Megasphaera elsdenii. The kinetic properties of both hydrogenases were studied and in addition, the properties of the three 4Fe-4S clusters of these enzymes no Ni is present were investigated. The redox properties of cytochrome-3 of D.vulgaris and flavodoxin of M.elsdenii were studied by their rates of reaction with the artifical electron donor the methyl viologen radical. Conversion of the enzyme from D.vulgaris from the oxygen sensitive form under working conditions into an oxygen- stable form was found to be feasible. The hydrogenase from M.elsdenii cannot be converted. We succeeded to produce hydrogen with a system of hydrogenase immobilized in reversed micelles by vectoral photosensitized electron transfer from thiophenol by means of a surfectant - Ru**2+ sensitizer. The efficiency of electron transfer from the mercury and glassy carbon electrodes to electron-transferring proteins was studied. Optimum electron transfer conditions were achieved.
Bibliographic Reference: EUR 9112 EN (1984) MF, 99 P., BFR 240, BLOW-UP COPY BFR 495 WRITE TO EUROFFICE, LUXEMBOURG, BP 1003.
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Record Number: 1989123041700 / Last updated on: 1987-01-01
Available languages: en