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Abstract

Eleven beagles were total body x-irradiated with 3 fractions of 6 Gy each ( 0.07 Gy/min ) at 48-hour intervals and were transfused with suspensions of fetal liver cells from DLA-identical or haplo-identical siblings, containing 9 to 93 thousand CFU-Gm per kg body weight. Bone marrow regeneration occurred rapidly in all animals, while the thymic remnants were fatty and hypoplastic. Lymph nodes were studied at autopsy between days 57 and 163 after transplantation. All lymph nodes showed a normal structure with numerous lymphocytes and germinal centers. Moderate to abundant plasma cells were found in the paracortex and medullary areas. Small to moderate numbers of phagocytes were also present. Granulopoiesis was noted in 7 cases and megakaryocytes were present in 2 cases. The number of lymphocytes in the blood was very low during the week after irradiation, started to increase the weeks thereafter and reached pretreatment values between days 75 and 101. Decreasing numbers of host lymphocytes were found in the blood and at the end of the observation period, all the metaphases were of donor type. Canine fetal liver cells are able to rescue lethally irradiated recipients and to restore lymph node morphology.

Additional information

Authors: CALVO W, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);PRUEMMER O, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);CARBONELL F, DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY);FLIEDNER T M DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY), DEPARTMENT OF CLINICAL PHYSIOLOGY AND OCCUPATIONAL MEDICINE, UNIVERSITY OF ULM (GERMANY)
Bibliographic Reference: 19TH ANNUAL MEETING OF THE EUROPEAN SOCIETY FOR RADIATION BIOLOGY, PRAHA (CZECHOSLOVAKIA), AUG. 26-30, 1985 WRITE TO CEC LUXEMBOURG, DG XIII/A2, POB 1907 MENTIONING PAPER E 32074 ORA
Availability: Can be ordered online
Record Number: 1989123119200 / Last updated on: 1987-01-01
Category: PUBLICATION
Available languages: en
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