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Abstract

We previously showed that radiation sensitive fibroblasts from ataxia-telangiectasia (A-T) patients sustain less G-2 delay after X-irradiation than normal fibroblasts (Scott and Zampetti-Bosseler, 1982). Caffeine is known to reduce the amount of X-ray induced delay in various mammalian cell types. Painter and Young (1980) proposed that A-T cells have an altered chromatin structure, similar to that of caffeine treated normal cells and that this results in a failure of A-T cells to delay their progression through the cell cycle to allow time for DNA repair. We now show that caffeine treatment after X-irradiation reduces G-2 delay in both A-T and normal cells. We confirm the results previously obtained on lymphocytes that caffeine potentiates the chromosome damaging effects of X-rays in both A-T and normal fibroblasts. These and other data suggest that the radiation responses of A-T cells and of caffeine treated normal cells are caused by different mechanisms.

Additional information

Authors: ZAMPETTI BOSSELER F UNIVERSITE LIBRE DE BRUXELLES, RHODE-ST. GENESE (BELGIUM) SCOTT D PATERSON LABORATORIES, CHRISTIE HOSPITAL AND HOLT RADIUM INSTITUTE, MANCHESTER (UK) , UNIVERSITE LIBRE DE BRUXELLES, RHODE-ST. GENESE (BELGIUM);PATERSON LABORATORIES, CHRISTIE HOSPITAL AND HOLT RADIUM INSTITUTE, MANCHESTER (UK)
Bibliographic Reference: MUTATION RESEARCH, VOL. 143 (1985), PP. 251-256
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