THE EFFECT OF CAFFEINE ON X-RAY INDUCED MITOTIC DELAY IN NORMAL HUMAN AND ATAXIA-TELANGIECTASIA FIBROBLASTS
We previously showed that radiation sensitive fibroblasts from ataxia-telangiectasia (A-T) patients sustain less G-2 delay after X-irradiation than normal fibroblasts (Scott and Zampetti-Bosseler, 1982). Caffeine is known to reduce the amount of X-ray induced delay in various mammalian cell types. Painter and Young (1980) proposed that A-T cells have an altered chromatin structure, similar to that of caffeine treated normal cells and that this results in a failure of A-T cells to delay their progression through the cell cycle to allow time for DNA repair. We now show that caffeine treatment after X-irradiation reduces G-2 delay in both A-T and normal cells. We confirm the results previously obtained on lymphocytes that caffeine potentiates the chromosome damaging effects of X-rays in both A-T and normal fibroblasts. These and other data suggest that the radiation responses of A-T cells and of caffeine treated normal cells are caused by different mechanisms.
Bibliographic Reference: MUTATION RESEARCH, VOL. 143 (1985), PP. 251-256
Record Number: 1989124019700 / Last updated on: 1987-01-01
Available languages: en