HYPOMETHYLATION OF REPAIR PATCHES IN HELA CELLS
In HeLA cells, under conditions where normal semiconservative synthesis is suppressed by hydroxyurea, the excision repair process after irradiation by UV results in a small amount of incorporation of nucleotides into nonreplicated DNA. By labelling the cytosine moieties of these repair patches, and measuring the ratio between cytosine and 5-methylcytosine, we have found that the level of methylation of cytosine in repair patches five hours after UV irradiation of the cells is about half of that observed in normal semiconservatively synthesized DNA.
Bibliographic Reference: MOLECULAR BIOLOGY REPORTS, VOL. 10 (1985), PP. 177- 182
Record Number: 1989124019800 / Last updated on: 1987-01-01
Available languages: en