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  • INSTANT IMAGES OF PROTOPLAST POPULATIONS THROUGH QUANTITATIVE CYTOLOGY - INITIAL CELLULAR UNIFORMITY AND RADIATION-INDUCED PROLIFERATIVE DISORDERS

Abstract

Cytophotometric measurements have proven to be of reasonable accuracy to monitor the cell cycle dynamics of relatively synchronized cell populations. The parameters are various, namely cellular areas, nuclear areas or relative nuclei DNA contents. The work was concerned by the application of this analytical method to stimulate protoplast populations, in cases where the classical isotope labelling method was intentionally avoided. The problem was tackled at two levels: a limitation coming from the possible confusion between haploid G2- and diploid G1-phases in frequently occurring mixoploid populations was acknowledged and assessed. Selection procedures were devised to overcome this difficulty by obtaining a satisfactory initial cytological uniformity. Then, the same approach was repeatedly followed for gamma irradiated protoplast populations, with the purpose to detect induced proliferative disorders. The method demonstrated its feasibility, leading to definite conclusions. In particular, a delay in the entrance in the post-irradiated S phase was shown and interpreted.

Additional information

Authors: MAGNIEN E, CEC BRUXELLES;FARAONI SCIAMANNA P CEC BRUXELLES, CEC BRUXELLES
Bibliographic Reference: 4. SIMPOSIO DINAMICO POPOLAZIONI, PARMA (ITALY), OCT. 22-24, 1981. ATTI DEL SIMPOSIO, PP. 199-207, 1984, PUBL. BY UNIVERSITA DI PARMA, ITALY
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