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Abstract

This project studies the application of polysaccharide degrading enzymes (cellulases, hemicellulases, pectinases) in the conversion of agricultural and horticultural raw materials, surplusses and wastes to fermentable sugar solutions. Several aspects were investigated: polysaccharide composition of the raw materials, commercial enzyme preparations for their liquefying and saccharifying capability, optimal reaction conditions of these enzymes, and the effect of pretreatment of the raw materials on enzyme action. The role of well characterized purified enzymes was studied in model experiments, especially cellulase, active on crystalline cellulose. Using commercial cellulolytic and pectolytic enzyme preparations, we liquefied and saccharified almost completely beet pulp, potato fibre and stubble turnips. A stirred batch was used for reactor extensive hydrolysis of their polysaccharides and for enzymic treatment of beet pulp a column hollow fibre reactor. Lignocellulosic materials gave mixed results. Preliminary experiments were carried out in the improvement of solid base fermentation of sugar beets. The cellulase preparation Maxazyme was studied in detail by fractionation and characterization of the different endoglucanases, exoglucanases and beta-glucosidases. Binding of the individual enzymes to crystalline cellulose was studied, and the endoglucanase - exoglucanase ratio for maximum synergism between the two enzymes.

Additional information

Authors: BELDMAN G, AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS);SEARLE VAN LEEUWEN M J F, AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS);VORAGEN A G J, AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS);ROMBOUTS F M, AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS);PILNIK W AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS), AGRICULTURAL UNIVERSITY, WAGENINGEN (THE NETHERLANDS)
Bibliographic Reference: EUR 9940 EN (1985) MF, 42 P., BFR 150, BLOW-UP COPY BFR 210, EUROFFICE, LUXEMBOURG, POB 1003
Availability: Can be ordered online
Record Number: 1989124108100 / Last updated on: 1987-01-01
Category: PUBLICATION
Available languages: en