MUTAGENIC TREATMENT OF POLLEN IN DIPLOIDS - SAMPLE SIZE, MUTATION RATE AND NUMBER OF LOCI INVOLVED
Pollen grains from a diploid pure line are submitted to mutagenic treatment. A metabolic pathway resulting in a given trait is assumed to be controlled by a set of n mutationally independent loci, each of which is liable to mutate towards a fully penetrant non-lethal recessive allele that interrupts the metabolic pathway when present in the homozygous state in a diploid. Untreated ovules from the same line are fecundated by treated pollen and yield M-1 plants which are selfed to produce the M-2 generation. A sample of M-1 individuals is drawn and the following problems are considered: The minimum sample size N that ensures, with a chosen minimum probability r, that at least one M-1 heterozygous carrier is present in the sample: the relationship between N, n, the number h of heterogeneous M-2 progenies and the mutation rate p per locus; the type of segregation ratio that is most likely to be observed in a heterogeneous M-2 progeny.
Bibliographic Reference: MUTATION RESEARCH, VOL. 174 (1986), PP. 205-206
Record Number: 1989124134800 / Last updated on: 1987-01-01
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