Community Research and Development Information Service - CORDIS

  • European Commission
  • CORDIS
  • Publications
  • HISTOCHEMICAL STUDIES ON THE EFFECTS OF LETHAL TOTAL BODY X-IRRADIATION ON THE PANCREAS OF DOGS RESCUED BY AUTOLOGOUS BONE MARROW TRANSPLANTATION

Abstract

Thirteen dogs were total body X-irradiated with three fractions of 6Gy delivered at 48 hr intervals and transplanted with cryopreserved autologous bone marrow cells. Pancreatic fibrosis was seen in all animals. In three of them, the exocrine pancreas was markedly reduced, they were cachectic and had to be euthanized 42, 48 and 89 days after irradiation. Their endocrine component was better preserved, specially the insulin and the pancreatic polypeptide secreting cells. In the nine long-term surviving dogs, various degrees of fibrosis were observed. Six of them showed abundant exocrine secretion. The Langerhans islets produced abundant insulin in all dogs. Secretion of glucagon, pancreatic polypeptide and somatostatin was also abundant in seven of the nine dogs. The histochemical and immuno histochemical methods proved to be useful to investigate the secretory activities of both exocrine and endocrine pancreas in irradiated dogs.

Additional information

Authors: MICHEL C, UNIVERSITY OF ULM (GERMANY);CALVO W, UNIVERSITY OF ULM (GERMANY);RAGHAVACHAR A, UNIVERSITY OF ULM (GERMANY);FLIEDNER T M UNIVERSITY OF ULM (GERMANY), UNIVERSITY OF ULM (GERMANY)
Bibliographic Reference: CELLULAR AND MOLECULAR BIOLOGY, VOL. 32 (1986), NO. 5, PP. 519-526
Record Number: 1989125042700 / Last updated on: 1987-07-01
Category: PUBLICATION
Available languages: en
Follow us on: RSS Facebook Twitter YouTube Managed by the EU Publications Office Top