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Abstract

AN OLIGOPEPTIDE FRACTION PURIFIED FROM THE EXTRACELLULAR COMPARTMENT OF BULL SEMEN AND STRONGLY INTERACTING WITH DNA WAS SHOWN TO HINDER MONONUCLEOTIDE POLYMERISATIONS TO DNA AND RNA IN VITRO. THE FRACTION, COLLECTIVELY CALLED SEMINAL PLASMA INHIBITOR, WAS ACTIVE IN THE ENDOGENOUS DNA AND RNA POLYMERASE REACTIONS OF THE NUCLEI FROM RAT HEPATOCYTES AND IN THE ANALOGOUS NUCLEOTIDE POLYMERISATIONS CATALYZED BY PURIFIED ENZYMES OF BACTERIAL ORIGIN. THE TYPE OF THE INDUCED INHIBITION WAS STUDIED USING THE RNA POLYMERASE FROM ESCHERICHIA COLI AS A REPRESENTATIVE NUCLEOTIDYL TRANSFERASE. IN THE ENZYMATIC POLYCONDENSATION OF MONONUCLEOTIDES, THE SEMINAL PLASMA INHIBITOR APPEARED TO EXERT ITS EFFECT MAINLY BY A COMPETITIVE INHIBITION FOR THE UTILIZATION OF DNA TEMPLATES WITHOUT SPECIFICITY WITH RESPECT TO THE SOURCE AND THE BASE SEQUENCE OF DNA. CONCAVITIES OF THE PLOTS OF V(O)/V(I) VERSUS THE AMOUNTS OF INHIBITOR IN THE NUCLEOTIDE POLYMERISING REACTION AND OF THE DIXON PLOTS IN THE ASSAYS OF RNA POLYMERASE FROM E COLI SUGGESTED THAT THE ISOLATED OLIGOPEPTIDE FRACTION CONTAINED MORE THAN ONE ACTIVE MOLECULAR SPECIES WITH DIFFERENTIAL EFFECTS AT LOW AND HIGH DOSES. PRELIMINARY RESULTS ON THE MICROHETEROGENEITY OF THE SEMINAL PLASMA INHIBITOR SUPPORTED THIS CONTENTION.

Additional information

Authors: LUGARO G, INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO;JRC-ISPRA;MORETTI R, INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO;JRC-ISPRA;CASELLATO M M, INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO;JRC-ISPRA;CAMPAGNARI F INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO, INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO;JRC-ISPRA;JRC-ISPRA, INSTITUTE OF HORMONE CHEMISTRY, CNR, MILANO;JRC-ISPRA
Bibliographic Reference: BIOCHIMICA ET BIOPHYSICA ACTA, VOL. 950, PP 420-428, 1988
Record Number: 1989126209000 / Last updated on: 1989-04-01
Category: PUBLICATION
Available languages: en