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Abstract

THE FLOW CYTOMETRY TECHNIQUES DEVELOPED AT JRC-ISPRA CAN BE A POWERFUL TOOL FOR AQUATIC ECOLOGY RESEARCH. THE CAPACITY FOR MULTIPARAMETER ANALYSIS ON SINGLE ALGAL CELLS ALLOW RESOLUTION OF CHANGES IN THE COMMUNITY STRUCTURE THAT WOULD BE OBSCURED BY TRADITIONAL ANALYSIS METHODS, IN WHICH AVERAGE CHARACTERISTICS OF SAMPLES ARE MEASURED. IN LABORATORY MULTI SPECIES TESTING, IT IS POSSIBLE TO CARRY OUT DAILY MEASUREMENTS OF THE CELL PARAMETERS: - SCATTERING PATTERN (INFORMATION ON SIZE, SHAPE, INTERNAL MORPHOLOGY, SPECIES DISTRIBUTION). - AUTOFLUORESCENCE (INFORMATION ON PIGMENT CONTENT, LIVING AND DEAD CELLS, PROKARYOTES FROM EUKARYOTES, SPECIES DISTRIBUTION). THE PARTITION IN REAL TIME OF SESTOMIC MATERIAL (PLANKTON PLUS TRIPTON) WITHIN WATER BODIES ON A DAILY BASIS BY SCATTERING AND INDUCED FLUORESCENCE MEASUREMENTS WAS DEMONSTRATED. THIS IS VITAL TO AN UNDERSTANDING OF RATE AND PATHWAY OF POLLUTANTS IN INLAND AND MARINE WATER BODIES.

Additional information

Authors: PREMAZZI G, JRC-ISPRA;BINDA S, JRC-ISPRA;ZILIO P, JRC-ISPRA;BUONACORSI G, JRC-ISPRA;BRAZZELLI A JRC-ISPRA, JRC-ISPRA
Bibliographic Reference: EUR 11450 EN (1988) MF, PP 22, ECU 4.00, BLOW-UP COPY, ECU 5.00, AVAILABILITY: EUROFFICE, LUXEMBOURG, BP 1003, GDL
Availability: Can be ordered online
Record Number: 1989126210200 / Last updated on: 1989-04-01
Category: PUBLICATION
Available languages: en