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Using a sequential mating procedure, the induction of specific-locus mutations by ethyl methanesulphonate (EMS) is reinvestigated in male mice. Doses of 175 mg/kg b.w. and 250 mg/kg b.w. of EMS induce gene mutations in the mating intervals 5-8 and 9-12 days post treatment. However, only the frequency of dominant lethal mutations increases with the dose, not the frequency of specific-locus mutations. This observation implies that with a higher dose of EMS a larger fraction of mutagenised spermatozoa and spermatids are selectively eliminated, leading to underestimation of the specific-locus mutation yield at high doses. EMS does not induce specific-locus mutations in spermatogonia.

Additional information

Authors: EHLING U H, GSF-Institut fuer Saeugetiergenetik, Neuherberg (DE);NEUHAUSER-KLAUS A, GSF-Institut fuer Saeugetiergenetik, Neuherberg (DE)
Bibliographic Reference: Article: Mutation Research, Vol. 227 (1989), pp. 91-95
Record Number: 199010111 / Last updated on: 1994-12-01
Original language: en
Available languages: en