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This report describes the development of an instrumental method for the detection and semi-quantification of sulphate-reducing bacteria in geothermal water, based on the principle of measurement of the culture duration in the exponential phase of growth required for reaching a predetermined metabolite concentration (sulphides) detected by electrochemical methods. Also included are a definition of the culture medium and of the detection system, validation on pure and mixed cultures (water from the natural environment), methods of dealing with the problems of cell sterilisation and techniques for maintaining the long-term efficiency of the electrochemical detection system. The method has the advantage of giving a response time of only 40 to 60 hours, instead of 8 to 30 days with a classical analysis. The development of an automatic on-line apparatus can be considered.

Additional information

Authors: COLIN F, Institut de Recherches Hydrologiques, Nancy (FR);JOURDAIN M J, Institut de Recherches Hydrologiques, Nancy (FR)
Bibliographic Reference: EUR 12524 FR (1990) 13 pp., MF, ECU 4
Availability: (2)
Record Number: 199010145 / Last updated on: 1994-12-01
Original language: fr
Available languages: fr