Matrix effects in FAB-MS and their use for MS/MS
MS/MS has been widely used to help solve mass spectrometric problems arising from spectra and sample complexity. When gas phase ionisation methods are used, only few parameters can be adjusted to maximise the information content of the spectra. When "soft" ionisation methods are used, the matrix composition can be selected to produce the best suited ions for a given type of experiment. Peptide sequencing by FAB-MS, using the Constant Neutral Loss scan is presented as an example. The various losses monitored (loss of CO, loss of H(2)O etc.), can be strongly enhanced by doping the matrix with inorganic salts. The anion has an important role in providing the optimum concentration of cation near the surface. The nature of the cation influences the type of induced fragmentation. This can be correlated with formation energies of the neutrals lost from the parent ions.
Bibliographic Reference: Article: Spectroscopy International Journal, Vol. 7 (1989) pp. 83-90
Record Number: 199010515 / Last updated on: 1994-12-01
Original language: en
Available languages: en