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Arsenic is widely distributed in the biosphere; it occurs in sea water at a 2ppb level. Marine animals and algae contain high amounts of arsenic by biotransformation and bioaccumulation. The toxicity of arsenic is dependent on its chemical form; inorganic species (+3 or +5 oxidation states) are more toxic than organic species. In order to evaluate the feasibility of the preparation and the certification of As-species in four materials (mussel tissue, fish, soil and sediment) several modern separation methods have been applied. An HPLC method has been used for the separation of the arsenite (AsO(2)-), arsenate (AsO(4)(3-)), monomethyl arsonic acid (MAA) and dimethylarsinic acid (DMA). The separation of these ionic species has been achieved using an anion-exchange column and UV detection. Furthermore, this separation is good enough to allow the collection of several fractions of eluate for energy dispersive X-ray fluorescence analyses (EDXRF). Capillary zone electrophoresis in a capillary silica column with an on-column UV detection (190 nm) has been used to resolve these arsenic anions with an acidic buffer. Good separations were demonstrated in a short time (15 minutes) with high efficiency.

Additional information

Authors: GRIEPINK B, CEC Bruxelles (BE);MAIER E A, CEC Bruxelles (BE);LEROY M J F ET AL., EHICS, Strasbourg (FR)
Bibliographic Reference: Paper presented: EUROANALYSIS VII, Vienna (AT), August 26-31, 1990
Availability: Text not available
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