Certification of an enzyme reference material for alkaline phosphatase (CRM 371)Funded under: FP2-BCR 4
A batch of lyophilised alkaline phosphatase (AP) has been prepared for use as an enzyme reference material. It is essentially free from contaminating enzyme activities. The kinetic properties of the preparation are very close to those of the enzyme present in human serum. The enzyme was partly purified from pig kidney to a specific activity of 400 U/mg of protein. The partly purified AP was lyophilised in a matrix containing bovine serum albumin (40 g/L), MgCl(2), ZnCl(2) and NaCl. The vial-to-vial variability with respect to the catalytic concentration of the final product was 0.008. The predicted annual relative loss of activity was less than 0.01% at -20 C and 0.04% at 4 C. This material was certified using the method proposed by IFCC. The certified alkaline phosphatase catalytic concentration in the reconstituted material was 254 U/L with a 0.95 confidence interval of +/- 6 U/L.
Bibliographic Reference: Article: Clinical Biochemistry, Vol. 24 (1991) pp. 159-168
Record Number: 199110784 / Last updated on: 1994-12-02
Original language: en
Available languages: en