Community Research and Development Information Service - CORDIS

Abstract

A batch of lyophilised alkaline phosphatase (AP) has been prepared for use as an enzyme reference material. It is essentially free from contaminating enzyme activities. The kinetic properties of the preparation are very close to those of the enzyme present in human serum. The enzyme was partly purified from pig kidney to a specific activity of 400 U/mg of protein. The partly purified AP was lyophilised in a matrix containing bovine serum albumin (40 g/L), MgCl(2), ZnCl(2) and NaCl. The vial-to-vial variability with respect to the catalytic concentration of the final product was 0.008. The predicted annual relative loss of activity was less than 0.01% at -20 C and 0.04% at 4 C. This material was certified using the method proposed by IFCC. The certified alkaline phosphatase catalytic concentration in the reconstituted material was 254 U/L with a 0.95 confidence interval of +/- 6 U/L.

Additional information

Authors: SCHIELE F, CNRS, Laboratoire du Centre de Médicine Préventive et Centre du Médicament, Nancy (FR);MULLER J, CNRS, Laboratoire du Centre de Médicine Préventive et Centre du Médicament, Nancy (FR);SIEST G, CNRS, Laboratoire du Centre de Médicine Préventive et Centre du Médicament, Nancy (FR);COLINET E, CEC Bruxelles (BE)
Bibliographic Reference: Article: Clinical Biochemistry, Vol. 24 (1991) pp. 159-168
Record Number: 199110784 / Last updated on: 1994-12-02
Category: PUBLICATION
Original language: en
Available languages: en
Follow us on: RSS Facebook Twitter YouTube Managed by the EU Publications Office Top