HPLC studies of aquatic humic compounds and complexes from the Drigg research site, Cumbria
This report describes an investigation into the applicability of high performance liquid chromatographic techniques for the separation of the complex mixtures of organic acids commonly found in groundwaters. At present there are no techniques available for assessing extraction efficiencies and changes that occur in organic matter on extraction and concentration, beyond simple determinations of UV absorbance and/or total organic carbon measurements. This work has shown that reverse phase ion-pair chromatography using a large pore stationary phase can be applied successfully to humic material in both natural and concentrated groundwater. The methodology separates the organic species into a number of well resolved components, the majority of which have a molecular weight of greater than 500 Dalton. Reverse phase HPLC was also investigated. It was concluded that any separation based on this mechanism is likely to suffer from poor inter-run reproducibility. Ion-suppression reverse phase was equally impracticable, requiring a mobile phase pH of less than 2 to obtain separation, which renders a silica based stationary phase unstable.
Bibliographic Reference: EUR 14082 EN (1992) 53 pp., FS, ECU 7.50
ISBN: ISBN 92-826-3811-1
Record Number: 199210330 / Last updated on: 1994-12-02
Original language: en
Available languages: en