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Abstract

Acid phosphatase has been prepared in an apparently pure state by affinity chromatography from human prostatic tissue. When dissolved in an acidic albumin solution, lyophilised and stored at -20 C for up to 2 years, no time-dependent loss of catalytic activity was detectable in the reconstituted material. Accelerated degradation tests also predicted complete stability. A preliminary distribution of the lyophilised preparation to 143 laboratories confirmed its robustness and demonstrated its potential usefulness as a calibrant to unify the results of different methods of measuring acid phosphatase activity.

Additional information

Authors: FRANCIS J M, Royal Postgraduate Medical School, Hammersmith Hospital, London (GB);MOSS D W, Royal Postgraduate Medical School, Hammersmith Hospital, London (GB);COLINET E, CEC Bruxelles (BE);CALAM D H, National Institute for Biological Standards & Control, Potters Bar (GB);BULLOCK D G, UK NEQAS for Clinical Chemistry, Wolfson Research Laboratories, Queen Elizabeth Hospital, Birmingham (GB)
Bibliographic Reference: Article: Annals of Clinical Biochemistry, Vol. 29 (1992) pp. 176-183
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