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Three peanut meal and two compound feed reference materials containing aflatoxin (B1) at different levels were prepared. The homogeneity of these reference materials was studied before and after packaging, while their stability was checked at four different temperatures with duplicate measurements for each temperature and time interval. Stability of aflatoxin B(1) was observed at -20 C and at +4 C. However, long-term stability studies demonstrated instability at +20 C and +37 C. The certification study came at the end of a series of intercomparison studies where the level of aflatoxin B(1) was decreased, more difficult matrices were chosen and the quality of the analytical procedures was improved to meet the Community Regulation on animal feedstuff. The results were evaluated on the basis of the following criteria: (i) recovery range 70-110%; (ii) repeatability of two recovery experiments, CV not greater than 15%, (iii) recovery to be within the normal laboratory range; (iv) recoveries to be at least consistent within the days of certification measurements. An intercomparison study of the supercritical fluid extraction was organised in parallel to the above certification study in order to use the certified values for aflatoxin B(1) from the above materials for an evaluation of this new extraction technique.

Additional information

Authors: BOENKE A, CEC Bruxelles (BE);WAGSTAFFE P J, CEC Bruxelles (BE);VAN EGMOND H P, National Institute of Public Health and Environment Protection, Bilthoven (NL)
Bibliographic Reference: Paper presented: Fifth International Symposium on Biological and Environmental RMs, Aachen (DE), May 11-14, 1992
Availability: Available from (1) as Paper EN 36824 ORA
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