Interlaboratory study of the IFCC method for alanine aminotransferase performed with use of a partly purified reference material
The paper presents the results of a study on performance of an enzyme reference material (ERM) for alanine transferase (ALT, EC 220.127.116.11), and also describes the corresponding International Federation of Clinical Chemistry (IFCC)-approved method in an interlaboratory trial involving 13 laboratories. The objectives of the study were to test the possibility of a partly purified ALT being an ERM and to assign a value to the material by using the IFCC method for ALT measurement. The ALT material was partly purified from pig heart and free of contaminating enzyme activities including that of aspartate aminotransferase. ALT catalytic concentration, kinetic properties, and long-term stability were examined, along with the effect of storage temperature on lyophilised ALT material. The transferability of the IFCC-approved method is shown.
Bibliographic Reference: Article: Clinical Chemistry, Vol. 38 (1992) No. 12, pp. 2365-2371
Record Number: 199310201 / Last updated on: 1994-11-29
Original language: en
Available languages: en