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The paper presents the results of a study on performance of an enzyme reference material (ERM) for alanine transferase (ALT, EC, and also describes the corresponding International Federation of Clinical Chemistry (IFCC)-approved method in an interlaboratory trial involving 13 laboratories. The objectives of the study were to test the possibility of a partly purified ALT being an ERM and to assign a value to the material by using the IFCC method for ALT measurement. The ALT material was partly purified from pig heart and free of contaminating enzyme activities including that of aspartate aminotransferase. ALT catalytic concentration, kinetic properties, and long-term stability were examined, along with the effect of storage temperature on lyophilised ALT material. The transferability of the IFCC-approved method is shown.

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Authors: SCHIELE F ET AL., Laboratoire du Centre de Médecine Préventive et Centre du Médicament, Nancy (FR);COLINET E, CEC Bruxelles (BE);ARZOGLOU P, University of Salonika (GR);BRETTSCHNEIDER H, Boehringer Mannheim GmbH, Penzberg (DE);CALAM D H, National Institute for Biological Standards and Control, Potters Bar (GB);CERIOTTI F, Istituto Scientifico, Milano (IT);FÉRARD G, Université Louis Pasteur, Strasbourg (FR);FREI J, CHU Vaudois, Lausanne (CH);HADJIVASSILIOU A, Metaxas Memorial Cancer Hospital, Piraeus (GR);HAFKENSCHEID J C M, Sint Radboudzienkenhuis, Nijmegen (NL);HORDER M, Odense University Hospital (DK);JAVIER GELLA F, Universitat Autonoma, Barcelona (ES);REJ R, Wadsworth Center for Laboratories and Research, New York (US);SCHMIDT E, Medizinische Hochschule, Hannover (DE);VASSAULT A, Hôpital Necker-Enfants Malades, Paris (FR);VIETTE V, Hôpital de la Chaux-de-Fonds (CH);FERARD G, Universite Louis Pasteur, Strasbourg (FR)
Bibliographic Reference: Article: Clinical Chemistry, Vol. 38 (1992) No. 12, pp. 2365-2371
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