Conjugated forms of salbutamol present in urine from salbutamol-treated veal calves
The use of beta-agonists as feed additives to improve the production of lean meat is not yet permitted in the European Communities. Nevertheless, these drugs are used illegally as production enhancers with the substances most often mentioned being clenbuterol and salbutamol. On behalf of the CEC's programme on measurement and testing (BCR), certified reference materials for beta-agonists analysis are under preparation. Urine reference material prepared from veal calves treated with clenbuterol and salbutamol will form these CRMs. The main metabolite of clenbuterol in urine is clenbuterol itself, whereas salbutamol is said to be found mainly under conjugated forms. The conditions of enzymic hydrolysis of urine from salbutamol-treated cattle were studied to completely release salbutamol from its conjugated forms. Radioimmunoassay (RIA) results, obtained after different types of enzymic hydrolysis with E. Coli beta-glucuronidase, Helix Pomatia juice and arylsulphatase in the presence of a specific inhibitor of beta-glucuronidase, indicated that salbutamol residues are present in urine mainly as glucuronide conjugated form. It follows that the conditions of enzymic hydrolysis and extraction of salbutamol residues should be standardised in the various laboratories involved in the future circular analysis of the reference material of salbutamol in urine.
Bibliographic Reference: Paper presented: EuroResidue II, Veldhoven (NL), May 3-5, 1993
Availability: Available from (1) as Paper EN 37409 ORA
Record Number: 199310580 / Last updated on: 1994-11-29
Original language: en
Available languages: en