Separation of nucleosides and nucleotide-3'-monophosphates by micellar electrokinetic capillary chromatography
Simultaneous separation of ionic and neutral compounds in micellar electrokinetic capillary chromatography (MECC) is a consequence of a combination of charge/mass ratios, hydrophobicity and charge interactions at the surface of the micelles. The type of resolution can be chosen mainly by modification of the buffer composition and pH. Reproducibility depends on the capillary tubing characteristics, operating conditions, and temperature effects. This paper reports on the different buffer compositions, pH, temperature, operating conditions, and capillary tubing treatments for the optimal separation and best reproducibility of deoxynucleotide-3'-monophosphates. The effects of different buffer compositions were tested when operating in a constant current mode at 3.8 micro amps.
Bibliographic Reference: Article: The Journal of Microcolumn Separations, Vol. 5 (1993) pp. 105-118
Record Number: 199310900 / Last updated on: 1994-11-29
Original language: en
Available languages: en