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Abstract

The Community Bureau of Reference (BCR) has undertaken a project to improve methodology and to prepare suitable certified reference materials for analytical quality control for the determination of ochratoxin A. The first phase of the project, an intercomparison of procedures for the determination of ochratoxin A in wheat at a level of approximately 13 microgram/kg, is described. The study involved 24 European laboratories which analysed a naturally contaminated wheat and a blank wheat sample (ochratoxin A content greater than 1 microgram/kg). The participants used a variety of procedures, including chloroform, methanol, toluene and ethyl acetate for extraction, and silica-, reversed phase- and immuno-affinity columns for clean-up. HPLC (one laboratory used TLC) was applied as the determinative step. Several performance characteristics were checked and the ochratoxin A content was determined. Recoveries were found to range from 25 to 100 %. The coefficient of variation from all the results calculated on the basis of peak height was 23 %. The study showed that the variation of results was influenced more by the clean-up step than by the extraction solvent. Some laboratories suffered significant day-to-day effects while others found difficulties with interfering peaks in the blank material.

Additional information

Authors: HALD B, Royal Veterinary and Agricultural University, Department of Veterinary Microbiology, Friedriksberg (DK);WOOD G M, Leatherhead Food Research Association (GB);BOENKE A, CEC Bruxelles (BE);SCHURER B, CEC Bruxelles (BE);FINGLAS P, AFRC Institute of Food Research, Norwich Laboratory (GB)
Bibliographic Reference: Article: Food Additives and Contaminants, Vol. 10 (1993) No. 2, pp. 185-207
Record Number: 199310923 / Last updated on: 1994-11-29
Category: PUBLICATION
Original language: en
Available languages: en
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