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The effects of fourteen metal ions (As(3+), As(5+), Cd(2+), Co(2+), Cr(3+), Cr(6+), Hg(2+), Li(+), Mg(2+), Mn(2+), Ni(2+), Se(4+), V(5+), VO(2+)) on the proliferation and differentiation in mouse B16 melanoma cells cultivated in vitro were analysed. Cell number assays, melanin, and protein measurements, a 3(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide reduction test (MTT survival test), and a clonal growth assay were performed. At 1.0 E-4 M, metal ions such as As(3+), As(5+), Cd(2+), Cr(6+), Se(4+), V(5+), VO(2+), and, to a minor extent, Li(+), Hg(2+), and Co(2+) significantly reduced the number of the B16 melanoma cells. For the same molar concentration, the order of the levels of cell toxicity of the metal compounds to B16 cells as measured by the MTT test was as follows: Hg(2+) greater than Cr(6+) = Cd(2+) greater than As(3+), As(5+) greater than V(5+), VO(2+) greater than Se(4+) = Ni(2+) = Co(2+) = Li(+). An increased synthesis of melanin in B16 cells was noted after incubation with Co(2+), Ni(2+), Cd(2+), and Li(+), whereas Se(4+) had, on the contrary, an inhibiting effect on melanogenesis.

Additional information

Authors: BAHBOUTH E, JRC Ispra (IT);SIWEK B, JRC Ispra (IT);SABBIONI E, JRC Ispra (IT);DE PAUW-GILLET M-C, University of Liège, Department of Histology and Cytology (BE);BASSLEER R, University of Liège, Department of Histology and Cytology (BE)
Bibliographic Reference: Article: Biological Trace Element Research, Vol. 36 (1993) pp. 191-201
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