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The rapid increase in interest in high performance liquid chromatography (HPLC) has shown no signs of diminishing in recent years. Where, for example, the elution behaviour of a given PAH mixture on C(18)-RP phases from different commercial suppliers is studied, the practising chromatographer most commonly uses the empirical approach to decide on chromatographic conditions. The application of a chemometric approach consisting of test systems and/or reference materials (RMs) for the characterisation of the performance of HPLC-columns is not very popular. However, efficient test systems and/or representative RMs may facilitate the characterisation and standardisation of the same stationary phases from different sources. This characterisation and standardisation should be based on the modified Van Deemter equation or Knox equation, the selectivity factor and on a definite preparation of the stationary phase together with a definite column packing procedure. A reference material might be one milestone of this chemometric approach. This RM may consist of a kit of two different reference solutions, one for the control of band-broadening and the other for judging selectivity, and one standardised C(18)-HPLC column as well. The various requirements and possibilities of such a proposed RM are explained and discussed.

Additional information

Authors: BOENKE A, CEC Bruxelles (BE)
Bibliographic Reference: Paper presented: 17th International Symposium on Column Liquid Chromatography, Hamburg (DE), May 9-14, 1993
Availability: Available from (1) as Paper EN 37697 ORA
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