Fraction collection after an optimized MECC separation of nucleic acid constituents
The MECC separation conditions were optimised to enable collection of the fractions for MS analysis. The different operational factors (buffer type, capillary treatment, temperature control, separation conditions) and their influence on quality of separation, resolution and efficiency have been investigated previously to reach a good reproducibility and repeatability. The optimisation was effected on a 50 microns I.D. and 50 cm length capillary as a good compromise between the loading capacity and the Joule heating effects. After verifying adequate loading capacity, fraction collection runs were performed. Samples were collected by electromigration into a fraction vial containing several microlitres of running buffer without SDS. The reinjection of the fractions collected revealed high recoveries (greater than 75 %). Fractions were further analysed by FAB-MS in order to investigate the possibility of analysis after a micellar separation.
Bibliographic Reference: Article: Journal of Chromatography, Vol. 638 (1993) pp. 363-373
Record Number: 199311216 / Last updated on: 1994-11-29
Original language: en
Available languages: en