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Abstract

This is an in vitro study of the effects of some selenite compounds on B16 melanoma cells in mice. The number of cells decreases by 55 % and 95 % at 10 micro-M and 100 micro-M following incubation with selenite and selenocistine (SeCy). It does not happen with selenate or selenometionine. The former produce a decrease of reduced glutation (GSH), larger for SeCy (30 % at 10 micro-M). Pre-incubation with GSH increases the toxicity of selenite, whilst GSH-peroxidase activity increases following incubation with selenite or SeCy. Controls in the absence of cells show that the increase mostly corresponds to glutation-reductase. Data suggest that selenite reacts with GSH to form Cy-Se(-), which in turn could produce radicals free from oxygen. All these could be species directly responsible for oxidising -tiol groups, for the inhibition of protein synthesis as well as for some gene mutation and antiproliferation effects.

Additional information

Authors: SERRA M A, JRC Ispra (IT);BLANCH N, JRC Ispra (IT);BAHBOUTH E, JRC Ispra (IT);SABBIONI E, JRC Ispra (IT)
Bibliographic Reference: Paper presented: XVIII Congreso Nacional de Bioquimica, San Sebastian (ES), September 28 - October 2, 1993
Availability: Available from (1) as Paper ES 37652 ORA
Record Number: 199311591 / Last updated on: 1994-11-28
Category: PUBLICATION
Original language: es
Available languages: es
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