Development of human endothelial cell linesFunded under: FP2-BRIDGE
Within the framework of the Community's biotechnology research programmes, groups of contractors were brought together to constitute ELWWs, with a view to facilitating the rapid circulation and exchange of information, data and materials, and increasing mobility among research staff. The purpose of this brochure is to illustrate the work of one such ELWW in the field of the development of human endothelial cell lines. Endothelial cells are the first cells to be exposed to foreign agents in the circulation and are particularly sensitive to toxic substances. The objective of the project was to produce one or more endothelial cell lines retaining differentiated functional properties but an indefinite life span with a view to their characterisation and use in pharmacological and toxicological tests. Progress is reported in the following areas: (i) the construction of retroviral vectors; (ii) the construction of plasmid vectors; (iii) the transfection of endothelial cells with constructs; (iv) the selection of oncogenes; (v) the optimisation of growth conditions; (vi) the establishment of functional assays. Teams and participants in the project are listed and a glossary of terms is also provided.
Bibliographic Reference: Report: EN (1992) 11 pp.
Availability: Available from the BRIDGE Programme Office, CEC, DG XII, 200 rue de la Loi, B-1049 Bruxelles (BE)
Record Number: 199330023 / Last updated on: 1994-11-29
Original language: en
Available languages: en