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  • COST 88 : Workshop on immunological and PCR-based methods to detect and quantify populations of Erwinia carotovora subsp. atroseptica on potato tubers


Blackleg is an important field disease of potatoes and is caused in temperate regions by a seed-borne bacterial pathogen, Erwinia carotovora subsp. atroseptica (Eca), which, together with E. carotovora subsp. carotovora (ECC), is also the main causal agent of tuber rotting in stores. The purpose of this workshop was to assess several methods which could be used to determine Eca tuber contamination, with particular emphasis on quantification of populations directly from potatoes. The following methods, developed in three COST 88 member countries, were selected: (i) viable count method; (ii) immuno magnetic cell separation; (iii) DOT-ELISA; (iv) immunofluorescent colony staining; (v) double antibody sandwich ELISA (DAS-ELISA); (vi) Staphylococcus aureus slide agglutination; (vii) PCR-based method. In this report each of the methods is described in detail, together with an algorithm of the steps involved, and the materials used in the project are listed.

Additional information

Authors: CEC, CEC Bruxelles (BE)
Bibliographic Reference: Report: EN (1992) 35 pp.
Availability: Available from the BRIDGE Programme Office, CEC, DG XII, 200 rue de la Loi, B-1049 Bruxelles (BE)
Record Number: 199330045 / Last updated on: 1994-11-29
Original language: en
Available languages: en