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  • The intensity of vanadium(V)-induced cytotoxicity and morphological transformation in BALB/3T3 cells is dependent on glutathione-mediated bioreduction to vanadium(IV)

Abstract

Cytotoxicity and morphological transformation have been studied in BALB/3T3 cells for ammonium vanadate [vanadium(V)] and vanadyl sulphate [vanadium(IV)] alone or in combination with diethylmaleate (DEM), a cellular glutathione (GSH)-depleting agent. The materials and methods used are described under the following headings: (i) chemicals, radiochemicals and counting the radioactivity; (ii) cell cultures and media; (iii) cytotoxicity assay; (iv) transformation assay; (v) oxidation state of vanadium by electron paramagnetic resonance. The results are presented with details of the intracellular bioreduction of vanadium, cytotoxicity and morphological transformation.

Additional information

Authors: SABBIONI E, JRC Ispra (IT);POZZI G, JRC Ispra (IT);DEVOS S, JRC Ispra (IT);FISCHBACH M, JRC Ispra (IT);PINTAR A, University of Milan, CNR Centre, Department of Inorganic and Metallo-organic Chemistry (IT);CASELLA L, University of Milan, CNR Centre, Department of Inorganic and Metallo-organic Chemistry (IT)
Bibliographic Reference: Article: Carcinogenesis, Vol. 14 (1993) No. 12, pp. 2565-2568
Record Number: 199410211 / Last updated on: 1994-11-28
Category: PUBLICATION
Original language: en
Available languages: en