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  • Induction of the human growth hormone gene placed under human hsp70 promoter control in mouse cells : A quantitative indicator of metal toxicity


An in vitro test method for general metal toxicity screening was designed, based on the cellular response to stress. The expression of a transfected human growth hormone gene sequence driven by the human heat-shock protein 70 promoter in NIH/3T3 cells was used as marker of noxious contact with metal compounds. 17 metals out of 31 tested induced this stress response system. According to the effective concentration and to the intensity of the response, three clusters of positive compounds emerged as strong, intermediate and weak inducers. These results correlate well with data from other in vivo and in vitro metal toxicity studies, including LD(50) in mice. The positive/negative compounds also fitted well with data from genotoxicity and carcinogenesis studies on metal salts.

Additional information

Authors: FISCHBACH M, Research Laboratories Dompè SpA, L'Aquila (IT);SABBIONI E, JRC Ispra (IT);BROMLEY P, CMU, Département de Génétique et Microbiologie, Genève (CH)
Bibliographic Reference: Article: Cell Biology and Toxicology, Vol. 9 (1993) No. 2, pp. 177-188
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