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  • Studies on the chromatographic behaviour and on the stability of hair dye precursors and hair dye couplers contained in oxidative hair dye formulations


Among the different methods of changing the colour of human hair, oxidative hair dyeing plays an important role. The formulations consist of a broad spectrum of organic compounds, most of which (the dye forming substances, the dye precursors and dye couplers) are of the aromatic type. The chromatographic behaviour of a selection of these products has been investigated. The separation was done by HPLC, using reverse-phase, UV and diode array detection. A separation procedure was established to separate and to identify precursors and couplers in model solutions according to their retention times and their UV spectra. In order to test the influence of light, temperature and antioxidants on the sample solutions, the changes in the chromatographic behaviour of the investigated substances were recorded as a function of these parameters.

Additional information

Authors: PEL E, JRC Geel (BE);BORDIN G, JRC Geel (BE);RODRIGUEZ A, JRC Geel (BE);WEIJLAND J W, Inspectie Gezondheidsbescherming, Keuringsdienst Van Waren, Enschede (NL)
Bibliographic Reference: Paper presented: 18th International J.F.S.C.C. Congress : The Cosmetic Image : A Mosaic of Biosciences, Venice (IT), October 3-6, 1994
Availability: Available from (1) as Paper EN 38312 ORA
Record Number: 199410907 / Last updated on: 1994-11-28
Original language: en
Available languages: en