HPLC/MS determination of diarrhetic shellfish poisoning (DSP) toxins as 4-bromomethyl-7-methoxycoumarin derivatives
An HPLC-method for determination of diarrhetic shellfish poisoning (DSP) toxins is described. The method is based on liquid extraction of the DSP toxins from contaminated algae and mussel material followed by an efficient clean-up procedure with silica gel before derivatisation. After pre-column derivatisation with 4-bromomethyl-7-methoxycoumarin (BrMmc), the reaction products are directly injected into an HPLC system with an RP18-column, isocratically eluted with acetonitrile/water (70/30) and quantified by fluorescence detection. The applied derivatisation reagent and the chromatographic conditions are also compatible with mass spectrometry.
Bibliographic Reference: Article: Proceedings of the 6th International Conference on Toxic Marine Phytoplankton, Nantes, 18-22 October 1993, France
Record Number: 199410927 / Last updated on: 1994-11-28
Original language: en
Available languages: en