A contribution to the study of the characteristics of several metallothioneins by high performance liquid chromatography
Four commercially available metallothioneins from rabbit liver (RL, RL I, RL II) and from horse kidney (HK) have been studied by size exclusion chromatography and by reverse-phase chromatography, to characterise their polymorphism. The isoforms were detected by UV at 250 nm. Separations by size exclusion show that the metallothioneins are partially dimerised, with molar rates of dimer ranging from 5 % for HK to 15 % for RL II. The four chromatograms obtained by reverse-phase exhibit a different morphology; RL separates into four main isoforms, RL I into two, RL II into three and HK into two. An attribution of the various peaks for the RL, RL I and RL II series is proposed. The comparable results obtained by the two methods allow the estimate that RL is composed of 20-30 % RL I and 70-80 % RL II.
Bibliographic Reference: Article: Canadian Journal of Chemistry, Vol. 72 (1994) pp. 1238-1245
Record Number: 199411128 / Last updated on: 1994-11-25
Original language: fr
Available languages: fr