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Abstract

This report describes the improved HPLC methods employed for the determination of DSP and PSP extracted from contaminated shellfish. For DSP determination, a column-switching HPLC system is used which permits the injection of DSP-containing extracts immediately after derivatisation with ADAM. This modified HPLC method allows a rapid and sensitive determination of DSP-toxins as ADAM derivatives with recovery rates near to 100 %. For PSP determination, the normal method employed is the Sullivan HPLC which uses an ion-pair chromatographic separation technique. The new method is based on HPLC separation of the underivatised PSP-toxins on an exchange resin, post-column oxidation with an electrochemical detector, and fluorescence detection.

Additional information

Authors: LUCKAS B, University of Hohenheim, Institute of Food Chemistry, Stuttgart (DE);HUMMERT C, University of Hohenheim, Institute of Food Chemistry, Stuttgart (DE);THIELERT G, University of Hohenheim, Institute of Food Chemistry, Stuttgart (DE);KIRSCHBAUM J, University of Hohenheim, Institute of Food Chemistry, Stuttgart (DE);BOENKE A, European Commission, Bruxelles (BE)
Bibliographic Reference: EUR 15339 EN (1994) 87 pp., MF, ECU 4
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