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Okadaic acid (OA), one of the most common diarrhetic shellfish poisoning (DSP) toxins, may cause, among other symptoms, gastrointestinal disorders. OA may occur in the digestive gland of shellfish. Sixteen European laboratories participated in an intercomparison study, within the framework of the Measurement and Testing programme (BCR) of the European Commission, in which extracts of the digestive gland of the mussel (Mytilis edulis) and simple solutions were analysed for their OA content. All participants used a reversed phase HPLC method and precolumn derivatisation to enhance fluorescence. Generally this was done with 9-anthryl-diazomethane (ADAM), either as crystals or prepared in situ. Optimal derivatisation conditions were established in ADAM. The stability of OA was checked and it was found that extracts and solutions proved less stable in time when stored at +4 C and +30 C rather than -18 C. A protocol of analysis was drawn up and compared with the results of a rat-bioassay. The HPLC method showed a good correlation.

Additional information

Authors: VAN TRIJP J M P, RIKILT-DLO, Wageningen (NL);HOLLMAN P C H, RIKILT-DLO, Wageningen (NL);TUINSTRA L G M T, RIKILT-DLO, Wageningen (NL);BOENKE A, European Commission, Bruxelles (BE)
Bibliographic Reference: EUR 15938 EN (1994) 57 pp., FS
Availability: Available from the BCR Programme Office, European Commission, DG XII, 200 rue de la Loi, B-1049 Bruxelles (BE)
Record Number: 199411368 / Last updated on: 1994-12-07
Original language: en
Available languages: en