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This report describes a collaborative study to certify a reference material of haemoglobinocyanide (HiCN) for use in standardisation of spectrometric measurement of haemoglobin. The material was derived from lysed bovine blood converted to HiCN by cyanide-ferricyanide reagent. Studies of storage conditions showed that freezing/thawing resulted in a progressive increase in absorbance at 540 nm, and a fall in the absorbance ratio. An increase in turbidity was shown by the absorbance at 750 nm being greater than 0.003. Accelerated degradation studies, including an Arrhenius plot on material stored at room temperature (20 C), 37 C and 45 C gave a prediction of stability for at least six years.

Additional information

Authors: LEWIS S M, Royal Postgraduate Medical School, Department of Haematology, London (GB)
Bibliographic Reference: EUR 16106 EN (1995) 54 pp., MF, ECU 4
Availability: (2)
Record Number: 199510318 / Last updated on: 1995-03-21
Original language: en
Available languages: en